Here is some supplementary material to the following interview
Full Interview. “DNA IS IN THE VACCINES.” 40 Trillion spikes in every shot! Dr Chris Shoemaker.
This article from Natural News serves as an introduction.
The Wuhan coronavirus (COVID-19) mRNA vaccines from Pfizer and Moderna have DNA contamination.Microbiologist Dr. Kevin McKernan has discovered “unacceptable levels” of double-stranded DNA plasmids floating around Pfizer and Moderna vaccines. Pharmaceutical and medical device R&D executive Sasha Latypova expounded on McKernan’s findings in an interview with “The Freedom Corner with Peter Sweden.”
Latypova said the DNA contamination is a significant problem because the replicable DNA plasmids can invade human cells and bacteria in the gut. This invasion can lead to the replication of antibiotic-resistant genes, sepsis, cancer and other health issues.
Experts at the World Council for Health (WCH) also confirmed that DNA contamination in mRNA vaccines is a global risk. They said the presence of replicable DNA plasmids in both the monovalent and bivalent vaccines is unacceptable, and called for an immediate halt to the COVID-19 vaccine program.
Besides the DNA contamination, McKernan also discovered the presence of Simian virus (SV40) promoters in Pfizer’s mRNA vaccine. The SV40 found in the vaccine is just a viral piece – not the whole virus. However, it still presents a risk of driving cancer.
SV40 was initially found in rhesus monkey kidney cells used to produce both inactivated polio vaccine (IPV) and oral polio vaccine (OPV.) When it was discovered to be an animal carcinogen, a law was passed in 1961 to prohibit its presence in vaccines. (Related: The true story of SV40, the cancer-causing virus hidden in polio vaccines.)
COVID-19 vaccines are disarming people’s immune systems
McKernan warned that there is a lack of quality control in the manufacturing process of Moderna and Pfizer vaccines, and it could pose risks such as anaphylaxis, blood clotting, antibiotic resistance, gene integration and long-term production of spike protein in the body.
Dr. Mark Trozzi, a Canadian physician and medical researcher, supported McKernan’s findings about the high concentrations of bacterial DNA in Pfizer and Moderna vaccines. In his interview with the New American, he stated that any traces of bacterial plasmids, including that from the Escherichia coli bacteria, should have been fered out in the final batches of mRNA COVID-19 vaccines.
“Whenever we see DNA contamination, like from plasmids, ending up in any injectable, the first thing people think about is whether there’s any E. coli endotoxin present because that creates anaphylaxis for the injected,” McKernan said.
Meanwhile, a study published in the Multidisciplinary Digital Publishing Institute journal showed that COVID-19 vaccine spike proteins inhibit the ability of damaged DNA to repair itself. “If DNA damage cannot be properly repaired, it will contribute to the amplification of viral infection-induced pathology,” the study authors warned, noting that immunodeficiency is a potential outcome.
“If you get an SV40 promoter in front of an oncogene, you will end up with a high expression of a gene that can drive cancer, it will be a very rare event, but you don’t need many of these cells to be hit with something like this for it to take off. SV40 actually plagued, granted it was the full viral genome, not just the promoter, but this has plagued previous vaccine programs. The polio vaccine is one of them that they were concerned, that this may have contributed to cancer from that vaccine. So, there’s a history of being concerned over SV40,” McKernan said.
Visit BadMedicine.news for more stories about the “dirty” COVID-19 vaccines.
Watch this video that explains how vaccines suppress DNA repair mechanisms in your cells.
A discussion between Dr.Kevin McKiernan and Dr.Sukharit Bhakdi
Why the COVID “mRNA” vaccines are actually DNA gene therapies that must be removed from the market
Sucharit Bhakdi, MD and molecular biologist named Kevin McKernan discuss how the so-called messenger RNA COVID vaccines are actually DNA gene therapies
This was an interview with Dr. Sasha Latypova
Turns out that most side effects come from the same batches.
Do you know what is really important when making a pharmaceutical product? That’s right, something called quality control. It’s really important to make sure that every product is the same in terms of dosage and quality, so that we know it works as intended.
But what happens if there is bad quality control and we get wildly different products? Well, it might look a little bit like what we have seen with the mRNA shots.
Because when it comes to side effects, it turns out that some batches of the mRNA shots had a lot more side effects than other batches. This would indicate different qualities of the product.
I spoke with Sasha Latypova about this, and she told me some very interesting things that you do not want to miss. Make sure to read to the very where you can find the link to watch my full video interview with Sasha Latypova. It is one of the most important videos you will watch.
”We still to date, we don’t know what is in those vials. The problem is because it’s completely unpredictable, but also the manufacturers are lying, the government is lying about the compositions” she told me.
THIS IS SHOCKING NEWS
Turns out that when people have tested the mRNA in the shots, there is mRNA there that is not supposed to be there!
Let that sink in.
The mRNA is supposed to be a certain length in order to code for the covid spike protein. However, they were finding mRNA in different lengths, meaning it won’t code for the spike protein. What will it code for in your body? Nobody knows.
People are getting injected with a product that hijacks their cells and instructs them to produce…something unknown.
But wait, it gets worse!
Sasha told me that the mRNA can also be carried around into the ovaries and sperm and can be carried into the embryo extra cellularly, possibly causing miscarriages. This is worrying stuff.
Did you know that not only is there unknown mRNA in the shots, but also they have found DNA in the shots. That is not supposed to be there at all!
The DNA that was found turns out to be a contaminant from the manufacturing process, in other words they did a bad job when producing the shots.
”It’s a huge problem because this is replication competent plasmid, it can then invade human cells, it can invade the bacterial cells that live in your gut. So they go into the bacteria they replicate there, they replicate antibiotic resistant genes…it can cause sepsis, it can cause cancer, all sorts of issues” Sasha told me in the interview.
This is pretty alarming stuff, and nobody is talking about it. That is why I sat down with Sasha to talk about it. Because unlike the mainstream media, I want the TRUTH.
What follows, from Dr. Paul Alexander is very technical
Does mRNA technology based COVID vaccine (Pfizer & Moderna) already contain DNA? Plasmids? Kevin McKernan?
Is the finding by Aldén et al. thus non-consequential, alarming as it was that mRNA was reverse transcribed; results indicate up-take of Pfizer into human liver cell line Huh7, leading to changes in LINE-1 expression & distribution; evidence Pfizer mRNA is reverse transcribed in 6 hours
Yet if there is already DNA contamination of the Pfizer & Moderna mRNA technology vaccines, then what are the implications? What exactly have we put into our bodies? Is out genetic material being re-programmed? Why? Was this really a binary bioweapon with stage one now delivered into us?
See Aldén et al. above:
Alexander COVID News-Dr. Paul Elias Alexander’s Newsletter is a reader-supported publication. To receive new posts and support my work, consider becoming a free or paid subscriber.
‘In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. We detected high levels of BNT162b2 in Huh7 cells and changes in gene expression of long interspersed nuclear element-1 (LINE-1), which is an endogenous reverse transcriptase. Immunohistochemistry using antibody binding to LINE-1 open reading frame-1 RNA-binding protein (ORFp1) on Huh7 cells treated with BNT162b2 indicated increased nucleus distribution of LINE-1. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. We also show that BNT162b2 mRNA is reverse transcribed intracellularly into DNA in as fast as 6 h upon BNT162b2 exposure.’
Yet see this substack by ANANDAMIDE (Nepetalactone Newsletter) about Pfizer and Moderna bivalent vaccines containing 20-35% expression vector and are transformation competent in E.coli.
Preamble- Follow on estimations of DNA/RNA contamination with alternative methods and more lots has reproduced contamination above the EMA limit but are less extreme than 20-35%. Please be certain to read the follow on substacks. These substacks look at Qubit readings and qPCR and found 43:1 – 161:1 Ratio of RNA:DNA in the Pfizer monovalent vaccines. Limitations- All lots evaluated to date are expired and this could lead to faster RNA decay than DNA decay. It is also possible, the Agilent Tape station DNA gels are staining some RNA and inflating the DNA estimates in the below study.
Previous work demonstrated that the Pfizer and Moderna bivalent vaccines are contaminated with the expression vectors used to manufacture the mRNAs. While EMA documents suggest these double stranded DNAs (dsDNAs) are linearized, no data is provided to quantitate this important step. Linear plasmids are less replication competent than circular plasmids. With early estimates of billions of potentially contaminating molecules of DNA, even 99% efficient linearization reactions could still leave millions of replication competent plasmids behind.
The initial sequencing of the mRNA vaccines did not sequence deeply enough to ascertain the completeness of the linearization reaction. The initial sequencing survey of these vaccines also utilized a directional RNA-Seq library construction method that contained actinomycin D. This additive is used to suppress DNA amplification in the RNA-Seq method as it intercalates with DNA and suppresses DNA dependent RNA polymerase activity of many polymerases. Thus the original 1:350 -1:3000 DNA:RNA read count levels likely under estimate the level of plasmid contamination present in the Moderna and Pfizer bivalent vaccines.
We explored several methods to properly assess the ratio of circular to linear DNA contaminating the vaccines. One method utilized RNase A treatment of the vaccine to remove the modified mRNA. These RNase A treated samples were then DNA purified. Once we had DNA from the vaccine, we could evaluate it with multiple orthogonal assays to triangulate a quantitative measurement of the nucleic acids.
Method 1) Transform vaccine derived DNA into Kanamycin sensitive E.coli cells and plate on Kanamycin selective plates. Circular plasmid will provide antibiotic resistance to E.coli and thus present colonies on Kan plates. Linear DNA is less likely to provide antibiotic selection.
Method 2 ) Design a multiplexed qPCR assay that targets the vector and the spike mRNA insert. These can be used to estimate the plasmid to mRNA ratio more quantitatively than actinomycin D based RNA-sequencing kits that bias the sequencing reads towards RNA.
Method 3) Electrophoresis of purified DNA and RNA from the vaccines to estimate the relative amounts and size of each nucleic acid.
Method 4) Whole genome shotgun sequence the DNA to afford higher coverage over the vector DNA than was obtainable with mRNA present in the library. The 2 million fold sequencing coverage over the vaccine only provided 500-4000 fold coverage over the vector. If we remove the RNA and focus the sequencers on the just the DNA, we can obtain 2 million fold coverage over the plasmid to look for rare circles.
The manufacturing of vaccine based mRNAs is best described by Nance et al.
This process can lead to residual plasmid DNA being left in the vaccine mRNAs. The method can also lead to truncated mRNA synthesis as seen in many RNA integrity (RIN plots) data listed as a concern by the EMA and TGA. The presence of E.coli based plasmids is a canary for Lipopolysaccharide (LPS or endotoxin) contamination. Whenever you see high levels of plasmid contamination derived from gram negative bacteria like E.coli, you should expect high levels of endotoxin contamination. Injecting endotoxin can lead to anaphylaxis and toxic shock syndrome.
The vector sequences discovered in previous sequencing work do not contain the long Poly A tracts described by Nance et al. This implies the poly-adenylation is performed after mRNA synthesis. This could be achieved with a polyA polymerase or a RNA ligase. The later method is more compatible with the unique poly-A tail described in Nance et al.
However, RNA ligases would also poly-adenylate truncated mRNAs from the original T7 polymerase manufacturing step. The polyadenlyation of truncated mRNAs would ensure the translation of truncated proteins. These questions should be further explored as poly A tracts are also notorious regions for assembly error. (See March 10th update below).
RNase A treatment of the Vaccines
RNase A cleaves both uracils and cytosines. N1-methylpseudouridine is known to be RNAse-L resistant but RNase A will cleave cytosines which still exist in the mRNAs. This leaves predominantly DNA for sequencing. Vaccine mRNA that was previously sequenced and discussed here, was treated at 37C for 20 minutes with 3ul of 20 Units/ul Monarch RNase A from NEB. The RNase reaction was purified using 1.5X of SenSATIVAx (Medicinal Genomics #420001). Sample were eluted in 20ul ddH20 after DNA purification. 15ul was used for DNA sequencing and 5ul used for transformation of E.coli.
Transformation of E.coli
50ul of NEBExpress competent E.coli cells (NEB#C2523I) were heat shocked at 42C for 20 seconds with 5ul of DNA (RNase treated vaccine) according to the manufacturers instructions. Cells were recovered in SOC for 1 hour with shaking at 37C. 200-800ul of SOC was plated on Kanamycin 25 plates (Teknova) and grown at 37C for 20 hours. Images were taken at both 20 hours and 44 hours.
Colonies were picked into 250ul of ddH20 and lysed with 2% LiDS at 37C for 5 minutes. DNA was purified with 1.5X Ampure and 2x 70% EtOH washes. DNA was run on a Agilent Tape station to assess gDNA and presence of plasmids. E.coli qPCR (Medicinal Genomics) was utilized to confirm the colonies were in fact E.coli.
Watchmaker Genomics fragment libraries were constructed from these E.coli colonies for further sequence confirmation of the transformation.
Whole genome shotgun of Pre-transformation RNase’d Vaccines.
15ul of the DNA (prior to E.coli transformation) was converted into sequence ready libraries using Watchmakers Genomics WGS library construction kit.
RT-qPCR of plasmid and insert of mRNA vaccines prior to transformation
Due to the use of actinomycin D in the RNA-seq first strand synthesis, RT-qPCR and qPCR was used to assess the insert to plasmid ratio. qPCR primers were designed using IDTs Primer Quest software targeting the Kanamycin gene in the plasmid (HEX) and the Spike protein in the plasmid and the mRNA (FAM). The primer coordinates are depicted in vector map produced by SnapGene.
CTAB/Chloroform/SPRI purification of Vaccines
Some variability in qPCR performance was noted with our LiDs/SPRI purification method of the vaccines. This left some samples opaque and is believed to be residual LNPs in the purification. A CTAB/Chloroform/SPRI isolation was optimized to address this and used for further qPCR and Agilent electrophoresis in Figures 10, 11 and 12. Briefly, 300ul of Vaccine was added to 500ul of CTAB (MGC solution A in SenSATIVAx MIP purification kit. #420004). The sample was then vortexed and heated for 5 minutes at 37C. 800ul of chloroform was added, vortexed and spun at 19,000 rpms for 3 minutes. The top 250ul of aqueous phase was collected and added to 250ul of solution B and 1ml of magnetic binding buffer. Samples were vortexed and incubated for 5 minutes and magnetically separated. The supernatant was removed and the beads washed with 70% Ethanol two times. Samples were finally eluted in 300ul of MGC elution buffer.
Previous RNA-Seq based estimates of the double stranded DNA contamination in the vaccines significantly under reported the magnitude of the contamination. Using qPCR and electrophoresis, we demonstrate the dsDNA contamination levels are 100 fold higher and imply trillions of DNA molecules per dose. The Pfizer DNA contamination ranges from 8.19-11.3 ng/ul with 23-28ng/ul of mRNA. The Moderna DNA contamination ranges from 7.5 – 9.5ng/ul with 25.7ng/ul – 55.9ng/ul of mRNA. This averages to 9.1ng/ul mean DNA concentration versus 33.4ng/ul mean RNA concentration.
This equates to 27.3% (9.1/33.4) of the nucleic acid in each vaccine being expression vector. This is several orders of magnitude over the the EMAs limit of 330ng/mg.
An unknown portion of these dsDNA contaminants are replication competent plasmids that can transform E.coli with a simple 20 second 42C heat shock treatment. These plasmids provide antibiotic resistance on LB-Kan plates and can be isolated from E.coli cultures. It is unlikely these plasmids will express spike protein in non-laboratory modified E.coli as the ribosomal signals in the vaccine mRNA are designed for mammalian translation. The T7 promoter is known to leak in mammalian cell lines and some laboratory E.coli genotypes but is not expected to leak in wild type E.coli. This may enable mRNA to be expressed from these plasmids in mammalian cells but unless the plasmids are integrated into the human genome, they are unlikely to be replicated to high copy number.
While bacteria are unlikely to express this spike protein, bacteria can replicate this plasmid and serve as a bactofection source for introduction of these mammalian expression plasmids to human cells.
Given the near equimolar contamination, studies evaluating the reverse transcriptase capacity of LINE-1 should be reconsidered. If each injection provides trillions of dsDNA contaminants, LINE-1 RT activity is not a necessary step for genome integration. The critiques of Alden et al focused primarily on the fact that LINE-1 is predominantly expressed in cancer cells lines and that the LINE-1 observation shouldn’t be extrapolated to patients. The vaccines are providing trillions of dsDNAs containing a potentially leaky T7 promoter encoding a spike protein with a Kozak consensus sequence. With these levels of contamination, RT activity from LINE-1 is not a prerequisite for genome integration. These data cannot inform on genome integration and further IRB reviewed deep sequencing work is required to address rare mosaic integration events in patients. Regardless of these hypothetical concerns, the dsDNA contamination exceeds the EMA specifications by several orders of magnitude and further scrutiny should be applied to the endotoxin levels and dsRNA levels in these vaccines.
These data are preliminary and further sequencing confirmation will be made publicly available once complete. Quantitative PCR assays are now available for detection of both Moderna and Pfizer monovalent and bivalent mRNA vaccines and their respective contaminating vector DNAs. This assay also detects the Janssen adenovirus spike sequence. These may be helpful for screening blood, semen, serum and other tissues for blood blanks and fertility clinics concerned with vaccine contamination.
While it is notable that the vector has a short poly A tract, we should wait until the ‘RNase A’ sequencing libraries are back before to we speculate too much about the manufacturing process ligating on poly A tracts. The RNase A libraries focus the sequencer on the DNA in the vaccine and allow us to examine those molecules without the 1000X higher signal from the mRNAs.
It is an important area of focus as it may be critical for interpreting the smeary western blots discussed by the EMA and others. Public sequence QC of the plasmids or the vaccine lots would greatly reduce public anxiety over this topic. We once enjoyed rapid data release policies in genomics with the Human genome project and the Bermuda accord. Some of this spirit persists today with over 6.7 millions SARs-CoV-2 genomes public in NCBI. Vaccine genomics, however defies this scientific sprit.
The vaccine sequences to date can be counted on 1 hand and can only be found in the depths of github or substack. A few accidental vaccine genomes exist due to sequencing patients plasma but there is no concerted effort to shine a spot lot on the variability of this novel genomic manufacturing process.
We have sequences of the viral genomes that involuntarily enter our body but none on the ones we can actually control via injection? The fidelity of the ExoN/RdRp polymerase gene in SARs-CoV-2 is higher than the error prone T7 polymerase with N1 methylpseudouridine suggesting sequence surveillance of the vaccine supply chain is more important than the sequencing the virus. This is a incoherent position given the RO of the vaccine traversed the globe via social media transmission as quickly as a biological virus.
Why caution is in order-
Poly A tracts are notorious locations for error in assembly as they have low complexity and often little signature to algorithmically organize. Sequences are assembled from pairs of 150 base pair reads. Imagine a jigsaw puzzle where all the pieces are the same color. You really only have the edge pieces to work with and in the case of sequencing a mixture of RNA and DNA you have conflicting edge pieces (at the poly A tail) that want to break the puzzle into two different puzzles.
The plasmid DNA sequences being circular, will disagree the most with the mRNA sequences right at the end of the poly A. So this may be an assembly artifact that DNA only sequencing clarifies. More to come on the next stack.
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Dr Paul Alexander’s Substack is HERE